The volatile anesthetic sevoflurane inhibits activation of neutrophil granulocytes during simulated extracorporeal circulation
Identifieur interne : 005818 ( Main/Exploration ); précédent : 005817; suivant : 005819The volatile anesthetic sevoflurane inhibits activation of neutrophil granulocytes during simulated extracorporeal circulation
Auteurs : Eckhard Schmid [Allemagne] ; Stefanie Krajewski [Allemagne] ; Daniel Bachmann [Allemagne] ; Julia Kurz [Allemagne] ; Hans Peter Wendel [Allemagne] ; Peter Rosenberger [Allemagne] ; Beverley Balkau [Australie, France] ; Karlheinz Peter [Australie] ; Klaus Unertl [Allemagne] ; Andreas Straub [Allemagne]Source :
- International immunopharmacology : (Print) [ 1567-5769 ] ; 2012.
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- Pascal (Inist)
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Abstract
Extracorporeal circulation (ECC) is an essential tool for the execution of cardiac operations. However, ECC is also associated with undesirable side effects. These include the induction of a systemic inflammatory response associated with leukocyte activation and cytokine release as well as potentially life-threatening complications. The volatile anesthetic sevoflurane has been reported to exert anti-ischemic and anti-inflammatory effects. We therefore investigated whether sevoflurane modulates the ECC-triggered inflammatory response. Heparinized human blood was circulated for 90 min in a normothermic (37 °C) ex vivo ECC circuit. An air-oxygen mixture was administered via an oxygenator in controls (n=5). Sevoflurane (2 vol.%) was added to the gas mixture in a second group (n = 5). At baseline and after 30, 60 and 90 min of ECC, blood samples were taken. In each sample whole blood counts were determined. Expression of the activation-indicating Mac-1 receptor on granulocytes and monocytes as well as leukocyte-platelet aggregate formation was measured in flow cytometry. Levels of the granulocyte activation marker PMN-elastase and of the cytokines IL-1β, IL-8 and TNF-α were analyzed using ELISA. ECC induced significant increases in Mac-1 expression on granulocytes (p<0.001) and PMN-elastase release (p<0.001). Sevoflurane decreased granulocyte Mac-1 expression during ECC (p<0.05) and inhibited the ECC-induced PMN-elastase release (p<0.05). Sevoflurane had no effect on whole blood cell counts, leukocyte-platelet aggregate formation and cytokine release during ECC. Sevoflurane inhibits granulocyte activation during ex vivo ECC and therefore has the potential to decrease the ECC-triggered inflammatory response. This promising finding warrants further investigation under clinical conditions.
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Intensive Care Medicine, University of Tübingen</wicri:noRegion></affiliation></author><author><name sortKey="Bachmann, Daniel" sort="Bachmann, Daniel" uniqKey="Bachmann D" first="Daniel" last="Bachmann">Daniel Bachmann</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Anesthesiology and Intensive Care Medicine, University of Tübingen</s1><s3>DEU</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>University of Tübingen</wicri:noRegion><wicri:noRegion>Department of Anesthesiology and Intensive Care Medicine, University of Tübingen</wicri:noRegion></affiliation></author><author><name sortKey="Kurz, Julia" sort="Kurz, Julia" uniqKey="Kurz J" first="Julia" last="Kurz">Julia Kurz</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Anesthesiology and Intensive Care Medicine, University of Tübingen</s1><s3>DEU</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>University of Tübingen</wicri:noRegion><wicri:noRegion>Department of Anesthesiology and Intensive Care Medicine, University of Tübingen</wicri:noRegion></affiliation></author><author><name sortKey="Wendel, Hans Peter" sort="Wendel, Hans Peter" uniqKey="Wendel H" first="Hans Peter" last="Wendel">Hans Peter Wendel</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Thoracic, Cardiac and Vascular Surgery, University of Tübingen</s1><s3>DEU</s3><sZ>5 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>University of Tübingen</wicri:noRegion><wicri:noRegion>Department of Thoracic, Cardiac and Vascular Surgery, University of Tübingen</wicri:noRegion></affiliation></author><author><name sortKey="Rosenberger, Peter" sort="Rosenberger, Peter" uniqKey="Rosenberger P" first="Peter" last="Rosenberger">Peter Rosenberger</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Anesthesiology and Intensive Care Medicine, University of Tübingen</s1><s3>DEU</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>University of Tübingen</wicri:noRegion><wicri:noRegion>Department of Anesthesiology and Intensive Care Medicine, University of Tübingen</wicri:noRegion></affiliation></author><author><name sortKey="Balkau, Beverley" sort="Balkau, Beverley" uniqKey="Balkau B" first="Beverley" last="Balkau">Beverley Balkau</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Baker IDI Heart and Diabetes Institute</s1><s2>Melbourne</s2><s3>AUS</s3><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Australie</country><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation><affiliation 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type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Unertl, Klaus" sort="Unertl, Klaus" uniqKey="Unertl K" first="Klaus" last="Unertl">Klaus Unertl</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Anesthesiology and Intensive Care Medicine, University of Tübingen</s1><s3>DEU</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>University of Tübingen</wicri:noRegion><wicri:noRegion>Department of Anesthesiology and Intensive Care Medicine, University of Tübingen</wicri:noRegion></affiliation></author><author><name sortKey="Straub, Andreas" sort="Straub, Andreas" uniqKey="Straub A" first="Andreas" last="Straub">Andreas Straub</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Anesthesiology and Intensive Care Medicine, University of Tübingen</s1><s3>DEU</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>University of Tübingen</wicri:noRegion><wicri:noRegion>Department of Anesthesiology and Intensive Care Medicine, University of Tübingen</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">International immunopharmacology : (Print)</title><title level="j" type="abbreviated">Int. immunopharmacol. : (Print)</title><idno type="ISSN">1567-5769</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">International immunopharmacology : (Print)</title><title level="j" type="abbreviated">Int. immunopharmacol. : (Print)</title><idno type="ISSN">1567-5769</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Activation</term><term>General anesthetic</term><term>Granulocyte</term><term>Inflammation</term><term>Inhibitor</term><term>Neutrophil</term><term>Sevoflurane</term><term>Volatiles</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Matière volatile</term><term>Sévoflurane</term><term>Inhibiteur</term><term>Activation</term><term>Neutrophile</term><term>Granulocyte</term><term>Inflammation</term><term>Anesthésique général</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Extracorporeal circulation (ECC) is an essential tool for the execution of cardiac operations. However, ECC is also associated with undesirable side effects. These include the induction of a systemic inflammatory response associated with leukocyte activation and cytokine release as well as potentially life-threatening complications. The volatile anesthetic sevoflurane has been reported to exert anti-ischemic and anti-inflammatory effects. We therefore investigated whether sevoflurane modulates the ECC-triggered inflammatory response. Heparinized human blood was circulated for 90 min in a normothermic (37 °C) ex vivo ECC circuit. An air-oxygen mixture was administered via an oxygenator in controls (n=5). Sevoflurane (2 vol.%) was added to the gas mixture in a second group (n = 5). At baseline and after 30, 60 and 90 min of ECC, blood samples were taken. In each sample whole blood counts were determined. Expression of the activation-indicating Mac-1 receptor on granulocytes and monocytes as well as leukocyte-platelet aggregate formation was measured in flow cytometry. Levels of the granulocyte activation marker PMN-elastase and of the cytokines IL-1β, IL-8 and TNF-α were analyzed using ELISA. ECC induced significant increases in Mac-1 expression on granulocytes (p<0.001) and PMN-elastase release (p<0.001). Sevoflurane decreased granulocyte Mac-1 expression during ECC (p<0.05) and inhibited the ECC-induced PMN-elastase release (p<0.05). Sevoflurane had no effect on whole blood cell counts, leukocyte-platelet aggregate formation and cytokine release during ECC. Sevoflurane inhibits granulocyte activation during ex vivo ECC and therefore has the potential to decrease the ECC-triggered inflammatory response. This promising finding warrants further investigation under clinical conditions.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Australie</li><li>France</li></country><region><li>Victoria (État)</li></region><settlement><li>Melbourne</li></settlement></list><tree><country name="Allemagne"><noRegion><name sortKey="Schmid, Eckhard" sort="Schmid, Eckhard" uniqKey="Schmid E" first="Eckhard" last="Schmid">Eckhard Schmid</name></noRegion><name sortKey="Bachmann, Daniel" sort="Bachmann, Daniel" uniqKey="Bachmann D" first="Daniel" last="Bachmann">Daniel Bachmann</name><name sortKey="Krajewski, Stefanie" sort="Krajewski, Stefanie" uniqKey="Krajewski S" first="Stefanie" last="Krajewski">Stefanie Krajewski</name><name sortKey="Kurz, Julia" sort="Kurz, Julia" uniqKey="Kurz J" first="Julia" last="Kurz">Julia Kurz</name><name sortKey="Rosenberger, Peter" sort="Rosenberger, Peter" uniqKey="Rosenberger P" first="Peter" last="Rosenberger">Peter Rosenberger</name><name sortKey="Straub, Andreas" sort="Straub, Andreas" uniqKey="Straub A" first="Andreas" last="Straub">Andreas Straub</name><name sortKey="Unertl, Klaus" sort="Unertl, Klaus" uniqKey="Unertl K" first="Klaus" last="Unertl">Klaus Unertl</name><name sortKey="Wendel, Hans Peter" sort="Wendel, Hans Peter" uniqKey="Wendel H" first="Hans Peter" last="Wendel">Hans Peter Wendel</name></country><country name="Australie"><region name="Victoria (État)"><name sortKey="Balkau, Beverley" sort="Balkau, Beverley" uniqKey="Balkau B" first="Beverley" last="Balkau">Beverley Balkau</name></region><name sortKey="Peter, Karlheinz" sort="Peter, Karlheinz" uniqKey="Peter K" first="Karlheinz" last="Peter">Karlheinz Peter</name></country><country name="France"><noRegion><name sortKey="Balkau, Beverley" sort="Balkau, Beverley" uniqKey="Balkau B" first="Beverley" last="Balkau">Beverley Balkau</name></noRegion><name sortKey="Balkau, Beverley" sort="Balkau, Beverley" uniqKey="Balkau B" first="Beverley" last="Balkau">Beverley Balkau</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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